SKU: 74977839933

VE-BEVS Insect Cell Line 10010 (1x10⁷ cells; frozen) (VE-CL-01) Enhanced expression of highly stable intracellular and secreted proteins.

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Description

VE-BEVS Insect Cell Line 10010 (1x10⁷ cells; frozen) (VE-CL-01) Enhanced expression of highly stable intracellular and secreted proteins.ParaTechs Vankyrin Enhanced Baculovirus Expression Vector System or VE BEVS technology enhances protein expression from the BEVS while reducing the cost of labor and materials. Vankyrin Enhanced Insect Cells (VE cells) are transgenic insect Spodoptera frugiperda (Sf9) cells that have been engineered to stably express the Campoletis sonorensis ichnovirus P vank 1 protein (Steele et al., 2017; Fath Goodin et al., 2006; Kroemer and Webb, 2006). Sf9

ParaTechs’ Vankyrin-Enhanced Baculovirus Expression Vector System or VE-BEVS technology enhances protein expression from the BEVS while reducing the cost of labor and materials. Vankyrin-Enhanced Insect Cells (VE cells) are transgenic insect Spodoptera frugiperda (Sf9) cells that have been engineered to stably express the Campoletis sonorensis ichnovirus P-vank-1 protein (Steele et al., 2017; Fath-Goodin et al., 2006; Kroemer and Webb, 2006). Sf9-derived VE-CL-01 cell line is suitable for the enhanced expression of highly stable intracellular and secreted proteins. The cells are sold in batches of 1×10⁷/ml cells in serum-free medium and need to be stored in liquid nitrogen. Please note this product ships on dry ice so additional shipping charges may apply. This product is intended for research purposes only.

Harmonized Tariff: 3002.90.5150

Advantages

  • Engineered monoclonal Sf9 cell line stably expresses a vankyrin gene
  • Mycoplasma free
  • Up to 8-fold increase in protein yield as compared to parental Sf9 cells
  • Prolonged longevity of cells after infection with a baculovirus compared to parental Sf9 cells
  • Increased baculovirus titer
  • Compatible with all conventional BEVS
  • Further enhancement can be achieved in combination with ParaTechs’ pAcVE transfer vectors (Product No. 20010, 20020 or 20030). View our VE-BEVS Insect Cell Line 10010 Complete Kits 30011, 30012 and 30013 for more information.
  • Reduced cost and labor
  • Expression of recombinant protein may need to be optimized by testing different time points and MOIs.


    Documents

    VE-BEVS Cell Line Instructions [PDF]

    VE-BEVS Cell Line FAQs [PDF]

    VE-BEVS Licensing

    Contract service for introducing the vankyrin gene into a custom insect cell line is available upon request.

    Patent information:
    VE-BEVS Cell Line United States Patent 7,842,493 [PDF]
    VE-BEVS Transfer Vector United States Patent 7,629,160 [PDF]

    Shipping Notes
    • Free Standard Shipping on $100+ Orders to the USA.
    • Except Preorder products are shipped in 48 hours.
    • Delivery to the USA:
    1. Standard Shipping : 3-10 business days
    • If time is of the essence, please consider selecting expedited delivery for faster service.
    Exchange/Return Notes
    • We offer a 30-day return/exchange service after receiving.
    • Final sale items are not eligible for returns or exchanges.
    • To process your return/exchange, please contact us at [email protected]
    • Please click here for more details>>> Return & Exchange Policy
    SKU: 74977839933

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    4.1 ★★★★★
    Based on 1334 reviews
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    Verified Purchase
    Mitch
    Los Angeles, US
    ★★★★★ 5
    Our German Sheppard loves these
    Size: Medium
    These cost a bit more than tennis balls, but they are so much nicer and longer lasting. For starters, they stay cleaner than tennis balls because they’re smooth rubber. Dirt won’t build up on them and if anything does stick, like grass or soil, it falls off once the dog slobber dries. They’re also thick, so they don’t fall apart or blow out like a normal tennis ball does in our dog’s jaws after 30 seconds. Our GS chomps on these like crazy and the only damage they’ve suffered is a crack that developed from the edge of the hole, but the crack is growing very slowly and none of these balls have totally failed yet. The balls do whistle when thrown ant high speed and that may help a dog track and locate it, but I’m not sure. Our neighbors hear the whistling too so it’s far from silent. Lastly the orange ball is easy to locate out in our yard, but the dark blue practically disappears.
    WAS THIS REVIEW HELPFUL?YesReportShare
    Reviewed in the United States on April 6, 2025
    C
    Verified Purchase
    Casey B
    Lake Worth, US
    ★★★★★ 5
    Great for smaller dogs
    Size: Small
    These two balls are perfect for the smaller mouthed dog that loves to play fetch. These balls are not only super durable (lots of teeth biting), but float in the baby pool we use for our miniature dachshunds. The value here is much better than you’d find anywhere else. The noise, if bitten hard enough, was “low” at best. Easy to spot/find if overthrown. Will definitely buy again once these are in bad repair; so far, so good-love these for my fur babies!
    WAS THIS REVIEW HELPFUL?YesReportShare
    Reviewed in the United States on October 8, 2024
    E
    Verified Purchase
    E. Schall
    West Palm Beach, US
    ★★★★★ 5
    My dogs favorite balls
    Size: Medium
    These are great for my dog who always has to have a ball in her mouth. Not too hard so I don't have to worry about her teeth. They last for ever. They do lose their shape a little over time (like I said, she always has a ball in her mouth) they become a little oval in shape. They still bounce, she still chases it and she still chews on it so I don't think she notices or cares. I've gotten her a bag full of these over time and I have only seen one that she chewed a chunk out.
    WAS THIS REVIEW HELPFUL?YesReportShare
    Reviewed in the United States on September 28, 2023
    K
    Verified Purchase
    Kimmie is happy
    Battle Creek, US
    ★★★★★ 5
    My Dachshunds Love These
    Size: Small
    These balls are durable, flexible material, highly chewable without being easily destroyed. I stuff them with Milkbone Maro Snacks, which are just the right size to twist into the holes in the ball. My dogs literally get an hour+ chewing and chasing entertainment from these. They chew them, they sometimes 'pop' out of their mouths, go rolling across the floor, dogs chasing them. This satisfies their urge to hunt prey. Dachshunds can easily tear this kind of stuff-it-toy apart. These have lasted for a long time without tearing or shredding. I would NOT recommend them for a dog much larger than a tweenie weenie. They're a bit on the small side and might be swallowed by a larger dog. It would be cool it they also came in a larger size (and probably ball wall thickness also) for the bigger dogs to enjoy. These stuff-it chew balls are better than the kong balls my dogs had before these. Highly recommend them.
    WAS THIS REVIEW HELPFUL?YesReportShare
    Reviewed in the United States on April 19, 2024
    L
    Verified Purchase
    LJ
    Bozeman, US
    ★★★★★ 5
    My puppy’s favorite fetch toy!
    Size: Small
    Update Aug 27, 2024: After 10 months, these are still his favorite ball. He’s obsessed with fetching and plays with them all day. They do bounce really high. Unfortunately, the small size aren’t available for sale anywhere anymore. I tried the medium size, and he has a bit of trouble hanging on to them, but he manages. Weirdly, he prefers the orange one, but he also plays with the blue one. My 7 month old Shih Tzu puppy loves to fetch, and these are his new favorites. They’re small enough to fit in his mouth, and they are made of hard plastic, so he’s able to hold on to them. They have a lot of bounce, and this adds to the fun for him. I highly recommend them.
    WAS THIS REVIEW HELPFUL?YesReportShare
    Reviewed in the United States on December 17, 2023

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