SKU: 61562638162

Porcine CD40L/TNFSF5 (Cluster of Differentiation 40 Ligand) QuickTest ELISA Kit

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Description

Porcine CD40L/TNFSF5 (Cluster of Differentiation 40 Ligand) QuickTest ELISA KitThe Porcine CD40L TNFSF5 (Cluster of Differentiation 40 Ligand) QuickTest ELISA Kit measures CD40L TNFSF5 in Porcine Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add

The Porcine CD40L/TNFSF5 (Cluster of Differentiation 40 Ligand) QuickTest ELISA Kit measures CD40L/TNFSF5 in Porcine Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap/Det Ab working solution into each well, then add the standards and pilot samples into individual wells. If the sample contains CD40L/TNFSF5, a capture antibody-CD40L/TNFSF5-biotin-detection antibody complex was formed. After incubation, unbound conjugates were removed by wash buffer. HRP-Streptavidin was added. After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of CD40L/TNFSF5 in the sample was calculated by drawing a standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 61562638162

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4.6 ★★★★★
Based on 137 reviews
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Georgia
Chelsea, US
★★★★★ 4
Very good for aggressive chewers
Color: Red, Blue, Green, Yellow
Finally found ball that my aggressive chewer doesn't destroy. Has loud squeaker however he broke after 4 days. Probably better, noise gone. Very sturdy and he loves. Also big so doesn't roll under furniture.
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Reviewed in the United States on January 25, 2025
S
Verified Purchase
Samantha Anthony
Cuba, US
★★★★★ 5
Durable
Color: Purple
I bought this for my 80lb 1yr American Bully male,who has made it his mission to test all the toys his dad and I have bought. Now I have 3 other American Bullies, siblings 3yr old 2 females/1male, who did the same at that age and I mastered toy shopping or so I thought. Lol. My new pup loves balls and this is the only one he firstly can't fit completely in his mouth and can't tear it apart. He has tired!
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Reviewed in the United States on May 6, 2026
T
Verified Purchase
TIFF
Whiting, US
★★★★★ 5
My Dogs Loved Them!
Color: Purple
My dogs loved these for playing fetch. They seem heavier duty than previous ones I have had. Beware of the incoming constant squeak sounds if your pup gets really into chewing it.
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Reviewed in the United States on May 8, 2026
K
Verified Purchase
Kingfam
Cuba, US
★★★★★ 5
Shockingly, good ball!
Color: Purple, Color: Purple
I have a Doberman. She is a ball and toy destroyer. She is the most obsessive retriever I’ve ever had. Her retrieving urge, and indefatigability, puts my golden retriever to shame. Because of this, we throw the ball all the time. She is primarily an indoor dog and we have to exhaust her energy and so throwing the ball is how this is done. I got these balls in an effort to try to find something that would last longer than a few days. I have now had these 2 balls for six weeks. They are both still alive! It literally blows my mind. When I bought them, we took bets on how long they would last. I gave it a week. I lost my bet. I got the extra large size. It’s perfect for the giant jaw span of a full grown Doberman. It squeaks, which is irritating to us, but what she loves. She can lay there and chew on it and still not destroy it. They’re very heavy ball and bounce, so you have to be careful throwing it in the house cause you could hurt breakables. Whoever said that these feel like they came from the dollar tree must have been talking about something else. They are thick and sturdy balls. UPDATE: it has now been THREE MONTHS and our Doberman STILL has not destroyed these 2 balls. We play with them many times each day. WORTH EVERY PENNY ADDITIONAL UPDATE: it’s now been nine months. Took this photo this morning. We still have the balls and have since ordered two more. She chews on it multiple times every day. There’s a little hole in it now and it doesn’t squeak anymore but it’s 98% intact and still essentially indestructible. These balls are worth gold.
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Reviewed in the United States on March 27, 2023
B
Verified Purchase
Bill_in_Louisiana
West Palm Beach, US
★★★★★ 3
The dog loves 'em
Color: Blue
The dog loves the balls, and they're the right size for my German Shepherd. Lost a couple of starts because they're not very durable, the "teeth" are easily broken off, so I am frequently finding "lost teeth" across the house; the squeakers also cannot hold up to my dog's aggressive chewing. I would still buy them again, as like I said, my dog love 'em!
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Reviewed in the United States on February 15, 2026

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